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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation - Quality control and quality assurance - Laboratory safety and regulatory compliance - Pre-analytical, analytical, and post-analytical processes |
| Immunology and Serology | 5-10% | - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders - Immune system principles and components - Infectious disease serology |
| Hematology | 20-25% | - White blood cell morphology and diseases - Red blood cell physiology and disorders - Hemostasis and coagulation testing - Routine and automated hematology procedures |
| Blood Banking (Immunohematology) | 15-20% | - Blood group systems and antigens - Compatibility testing - Transfusion reactions and adverse events - Blood components and storage - Antibody detection and identification |
| Microbiology | 15-20% | - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility - Identification of pathogenic microorganisms - Specimen collection and culture techniques |
| Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases |
| Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Urine formation and composition - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Which of the following is most likely to interact with arterial walls, leading to deposition of cholesterol, and initiating or worsening atherosclerosis?
- A. Small dense LDL
- B. LDL phenotype 'A'
- C. Large buoyant LDL
Correct Answer: A 🗳️
The cell depicted with the arrow in this image is an atypical (reactive) lymphocyte. These cells are common found in certain viral infections, especially infectious mononucleosis. Notice the larger size and abundant cytoplasm present in this lymphocyte. There is also apparent vacuoliation which is a key feature of atypical lymphocytes. The chromatin pattern of this cell as well as the overall shape, color and size rules out the monocyte, macrophage, and mesothelial cell choices.
A patient with an infectious mononucleosis infection presents in the emergency room. Physicians order a spinal tap which is immediately sent to the laboratory for review. Please identify the cell in the image below from this patient's cerebrospinal fluid sample.
- A. Reactive Lymphocyte
- B. Mesothelial Cell
- C. Monocyte
- D. Macrophage
Correct Answer: A 🗳️
Haemophilus influenzae is both X and V factor dependent for growth, and is the most common cause of obstructive laryngitis and epiglottitis. H. parainfluenzae is only V-factor dependent and causes only mild pharyngitis, rarely obstructive in nature. H. aphrophilus can be part of the normal upper respiratory flora in humans, does not cause pharyngitis, and is not X or V-factor dependent for growth. Although H. haemolyticus is both X and V-factor dependent, it is beta hemolytic on blood agar and is generally considered non pathogenic.
Acute obstructive epiglottitis, both in adults and children, is caused by the bacterial species shown in this split screen photograph. The colonies recovered on chocolate agar (upper frame) required both hematin and NAD, (as shown by colony growth only between the X and V strips in the lower frame). The most likely identification is:
- A. Haemophilus parainfluenzae
- B. Haemophilus influenzae
- C. Haemophilus haemolyticus
- D. Haemophilus aphrophilus
Correct Answer: B 🗳️
Synovial fluid is actually quite viscous and does not have the same consistency as plasma. The reason for its thicker consistency is to provide cushion and reduce friction between the articular cartilage of synovial joints during movement.
Urinalysis & Other Body Fluids
All of the statements below about synovial fluid are true EXCEPT:
- A. joint fluid resembles plasma in viscosity
- B. arthrocentesis is performed as a means to evaluate arthritis and other joint disorders
- C. joint fluid has approximately the same glucose as plasma
- D. synovial fluid is present only in movable joints
Correct Answer: A 🗳️
The part of the microscope that holds the objective lenses is called the _______________.
- A. revolving nosepiece
- B. condenser
- C. fine adjustment
- D. objective
Correct Answer: A 🗳️
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